|
KMID : 1137820220430040193
|
|
의공학회지
2022 Volume.43 No. 4 p.193 ~ p.198
|
|
|
Enhancement Characteristics of Gadolinium Contrast Agent in the Rat Inner Ear Perilymph through CSF microcirculation
|
|
Kim Min-Jung
Lee Sang-Yeol
Lee Hui-Joong
Lee Tae-Kwan
Chang Yong-Min
|
|
|
Abstract
|
|
|
|
Contrast enhanced magnetic resonance imaging using gadolinium-based contrast agent (GBCA) is a very useful in vivo technique to visualize the inner ear pathology including endolymphatic hydrops. Although systemic intravenous (IV) administration can visualize the perilymph space, the visualization was possible by indirect passage of contrast agent through blood?perilymph barrier. All animal experimental procedures were performed under anesthesia with 5% isoflurane. Lipopolysaccharide (LPS) was instilled into the left tympanic cavity through the tympanic membrane using a sterile 27gauge needle to induce hydrops model. Tucker-Davis Technologies system was used to measure Auditory Brainstem Responses (ABRs). For intracerebroven- tricular (ICV) administration, 25 μmol of GADOVIST (Bayer, Berlin, Germany) was used and diluted GADOVIST injection was 10 μl. MR imaging was acquired with a 9.4 Tesla MRI scanner. Transmit- receive volume coil with 40 mm inner diameter and 75 mm out diameter was used. ICV administration well demonstrated the strong enhancement along the cerebrospinal fluid (CSF) microcirculation pathway including CSF fluid in the subarachnoid space and CSF space of the inner ear structures. On the other hand, IV administration showed no contrast enhancement along the CSF microcirculation pathway and showed weak enhancement in the inner ear structures. In case of rat hydrops model, ICV administration showed that the reduced contrast enhancement in the perilymph space of the hydrops induced inner ear compared to the contrast enhancement in the perilymph space of the normal inner ear. New systemic ICV administration method provide contrast enhancement of GBCA in the inner ear through CSF micro- circulation pathway.
|
|
|
KEYWORD
|
|
|
Intracerebroventricular administration, Gadolinium-based contrast agent, Endolymphatic hydrops, CSF
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|